Pharmacological action research
on Anticancer of China No. 1 Tian Xian Liquid )
Tianjin Institute of Medical Science Research
Tong Hua Chang Bai Shan Institute of Medicine, Jilin
Province
Research has identified that "CHINA
NO. 1 Tian Xian Liquid" has a significant
inhibition on mice S120, "Ai’s"
solid carcinoma, Lewis’ lung cancer, proventriculus
scute cancer and living nude mouse human intestine
mucinous adenocarcinoma.
Antineoplastic action of "China No.
1 Tian Xian Liquid"
Experiment with animal : Mice used are from Kunmin,
provided by units such as the Tianjin Medical College,
Consisting both male and female, weighing between
18 ~ 22g.
Nude mice were provided by Beijing Institute of
Organism Inspection, male weights between 20 ~ 27g,
the background of nude mice is BALB/C-nu/nu and
the heredity background is NIH.
Tumor strain : Mouse sarcoma S180, "Ai’s"
solid and ascites carcinoma, mouse proventriculus
scute cancer, Lewis’ lung cancer, mouse leukemia
L1210.
Human intestine mucinous adenocarcinoma : Introduced
by pathologic anatomy teaching and research section
of Beijing Medical College, grafted in the subcutaneous
layer of the back of the nude mouse as a model.
Medicine : "China No. 1 Tian Xian
Liquid" provided by Tong Hua Chang
Bai Shan Institute of Medicine, Jilin Province,
an oral administration Liquid of traditional Chinese
medicine.
Experimental Method : An ordinary screening method
for tumor strain according to the procedure regulated
by the National Anticancer Medicine Conference 1978.
The results are shown in Table 1.
The human cancer inhibition experiment was proceeded
according to following the procedures. First, let
the steriled operating bench ventilate for over
half an hour, place the mouse cage (equipped with
bacterial filter) onto the operating bench after
sterilizing it twice with 75% alcohol. Put the tumor
source nude mouse to death by pulling its cervical
vertebrae. Remove tumor tissue and cut it into small
pieces of 0.3 ~ 0.4 mm in size. Vaccinate tumor
tissue into the subcutaneous layer of the back of
each mouse with #7 puncture needle. After vaccinating
the human tumor tissue for 30 days, choose 24 nude
mice with similar size tumor and divide them into
4 groups, that is : the Control Group, "China
No. 1 Tian Xian Liquid" Group, 5FU Group, and
SJAMP Group. Of which, the Control Group consisted
of 9 mice and 5 mice for the rest of the groups.
Before treatment, the Control Group has an average
tumor diameter (longitudinal diameter + transverse
diameter /2) of 8.10 mm, the "China
No. 1 Tian Xian Liquid" Group has
an average tumor diameter of 8.6 mm. The therapeutic
dose of "China No. 1 Tian Xian Liquid"
is 2.5 ml/kg, gavaged once a day for 21 days. Put
all the animals of each group to death 24 hours
after the last administration. Cut out the tumors
and weigh them, and weigh each mouse too. Calculate
the inhibition rate based on the following formula.
Tumor Inhibition rate % = [(Average tumor weight
of control group – Average tumor weight of
treated group) / Average tumor weight of control
group] * 100%
Result of experiment : Among the 6 spectra of tumor
used, we have discovered that "China No. 1
Tian Xian Liquid" has better effect for solid
tumor especially for proventriculus scute cancer,
but is ineffective for leukemia and ascitic type
tumor. The results are shown in Table 1.
Go back to TOP.
| Tumor Strain |
Number of Animal |
Dosage g/kg |
Form of application and no. of
times |
Inhibition rate % or life prolongation
rate |
P Value |
| S180 |
10 |
2 |
PO12 |
35.6 |
|
| |
10 |
5 |
PO12 |
48.3 |
<0.05 |
|
10 |
Control
Group |
PO12
|
|
|
| "Ai's" solid carcinoma |
9 |
2 |
PO6
|
54.9
|
<0.01 |
| |
9 |
5 |
PO6
|
51.46 |
<0.01 |
|
9 |
Control
Group |
PO6
|
|
|
| Lewis' lung
cancer |
5 |
2 |
PO8
|
46.9 |
<0.05 |
| |
5 |
5 |
PO8
|
36.4 |
>0.05 |
|
5 |
Control
Group |
|
|
|
| Proventriculus scute cancer |
6 |
2 |
PO14 |
36.5 |
<0.01 |
| |
6 |
5 |
PO14 |
57.8 |
<0.01 |
|
6 |
Control
Group |
PO14 |
|
|
| "Ai's" Ascites carcinoma |
10
|
2 |
PO6 |
7.5 days |
|
| |
10
|
5 |
PO6 |
3.0 |
|
| 10
|
Control
Group |
PO6 |
7.1 |
|
| L1210 |
6 |
2 |
PO8 |
8.2 days |
|
| |
6 |
5 |
PO8 |
8.4 |
|
|
8 |
Control
Group |
PO8 |
8.0 |
|
Table 1. The antitumor action of "China
No. 1 Tian Xian Liquid"
It also has definite therapeutic action on human intestine mucinous adenocarcinoma, inhibition rate reaches up to 48.06%, P<0.05, please refer to Table 2.
| Group |
Animal's serial number |
Dosage, form of application and no. of times |
Weight of animal |
Average diameter of tumor (mm) at the beginning
of experiment |
Weight of tumor (g) at the end of experiment |
Inhibition rate for tumor |
p Value |
| Control Group |
|
|
Beginning |
End |
|
|
|
| 1 |
"0" |
23.5 |
20.0 |
9.0 |
1.10 |
| 2 |
0.5% between Liquid |
24.0 |
22.0 |
6.7 |
0.50 |
| 3 |
0.2ml / day |
24.5 |
23.5 |
6.0 |
1.95 |
| 4 |
Total 21 days |
23.7 |
20.0 |
5.3 |
1.60 |
| 5 |
|
22.5 |
22.0 |
7.3 |
0.80 |
|
| 6 |
24.0 |
20.0 |
10.0 |
1.70 |
| 7 |
24.5 |
19.0 |
9.4 |
0.70 |
| 8 |
23.0 |
20.5 |
10.8 |
2.20 |
| 9 |
24.0 |
24.0 |
8.4 |
1.10 |
| Average value |
|
23.7 |
21.2 |
8.1 |
1.29 |
| "CHINA NO. 1 TIAN XIAN LIQUID" |
1 |
"0" |
22.5 |
21.5 |
8.0 |
0.90 |
| 2 |
2.5g/kg, once a day |
26.0 |
23.5 |
9.4 |
0.85 |
| 3 |
Total 21 days |
20.0 |
19.0 |
7.7 |
0.40 |
| 4 |
|
20.0 |
18.5 |
8.0
|
0.90 |
| 5 |
|
20.0 |
19.0 |
8.2
|
0.30 |
| Average value |
|
21.7 |
20.3 |
8.06 |
0.67 |
48.06 |
<0.05 |
Go back to TOP.
Table 2. The inhibition of "China
No. 1 Tian Xian Liquid" on nude mice
human intestine mucinous adenocarcinoma.
From table 2, it is clear the "China
No. 1 Tian Xian Liquid" has definite
therapeutic action on human intestine mucinous adenocarcinoma.
Although the animal’s weight has somewhat
decreased, so have the ones in the control group.
All the animals are still surviving, which indicated
that taking the medicine continuously for 21 days
under that dosage, there is no toxic reaction in
the animals. Summarizing the experimental results
on animal tumor and human cancer, indicated that
"China No. 1 Tian Xian Liquid" has noticible
effect on digestive system tumor and lung cancer.
back to top
